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Janaki Pathi, P.
- The Estimation of Sapropterin Dihydrochloride in Tablet Dosage form by RP-HPLC
Authors
1 Analytical Department, Vishnu Chemicals Limited, Hyderabad, IN
2 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 2, No 4 (2012), Pagination: 110-113Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Sapropterin Dihydrochloride in tablet dosage form. An XTerra(R) C18 analytical column (250x4.6 mm, 5 μm particle size) with mobile phase consisting of mixture of buffer 0.02M Ammonium Acetate in water and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 238 nm. The retention time was 2.9 min. The detector response was linear in the concentration of 20-120 mcg/mL. The respective linear regression equation being y= 3234.6x-3233.6. The limit of detection and limit of quantification was 0.01mcg/mL and 0.03mcg/mL respectively. The percentage assay of Sapropterin Dihydrochloride was 99.4%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Sapropterin Dihydrochloride in bulk drug and in its pharmaceutical dosage form.
Keywords
Sapropterin Dihydrochloride, RP-HPLC and Tablets.
- The Estimation of Cefquinome Sulphate in Suspension Form by RP-HPLC
Authors
1 Analytical Department, Vishnu Chemicals Limited, Hyderabad, IN
2 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 2, No 2 (2012), Pagination: 33-35Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Cefquinome Sulphate in suspension form. An XTerra(R) analytical coloumn (C18, 250 x 4.6 mm, 5 μm particle size), with mobile phase consisting of mixture of buffer 0.02M Ammonium Acetate in water and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 234 nm. The retention time was 6.06 min. The detector response was linear in the concentration of 4 - 48 mcg/mL. The respective linear regression equation being y=849408x-849408. The limit of detection and limit of quantification was 0.01mcg/mL and 0.03mcg/mL respectively. The percentage assay of Cefquinome Sulphate was 99.4%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Cefquinome Sulphate in bulk drug and in its suspension form.
Keywords
Cefquinome Sulphate, RP-HPLC and Suspensions.- The Estimation of Epalrestat in Tablet Dosage Form by RP-HPLC
Authors
1 Analytical Department, Vishnu Chemicals Limited, Hyderabad, IN
2 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 2, No 2 (2012), Pagination: 49-51Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Epalrestat in tablet dosage form. An XTerra(R) C18 analytical column (250x4.6 mm, 5 μm particle size) with mobile phase consisting of mixture of buffer (0.03M Potassium Dihydrogen phosphate in water at pH 3.2 with ortho-phosphoric acid) and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 294 nm. The retention time was 15.9 min. The detector response was linear in the concentration of 20- 120 mcg/mL. The respective linear regression equation being y=3818.8x-3819. The limit of detection and limit of quantification was 0.005mcg/mL and 0.015mcg/mL respectively. The percentage assay of Epalrestat was 99.3%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Epalrestat in bulk drug and in its pharmaceutical dosage form.
Keywords
Epalrestat, RP-HPLC and Tablets.- The Estimation of Palonosetron Hydrochloride in Parenterals by RPHPLC
Authors
1 Analytical Department, Vishnu Chemicals Limited, Hyderabad, IN
2 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034, IN
Source
Asian Journal of Pharmacy and Technology, Vol 2, No 2 (2012), Pagination: 77-79Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Palonosetron Hydrochloride in dosage form. An XTerra(R) C18 analytical column (250x4.6 mm, 5 μm partical size) with mobile phase consisting of mixture of buffer 0.03M Potassium Dihydrogen Orthophosphate in water and pH adjusted to 3.20 with Orthophosphoric acid and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 242 nm. The retention time was 10.9 min. The detector response was linear in the concentration of 5-30 mcg/mL. The respective linear regression equation being y= 3644.3x-3644.7. The limit of detection and limit of quantification was 2.5ng/mL and 7.5ng/mL respectively. The percentage assay of Palonosetron Hydrochloride was 99.6 %. The method was validated by determining its accuracy, precision and linearity.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Palonosetron Hydrochloride in bulk drug and in its pharmaceutical parenterals form.
Keywords
Palonosetron Hydrochloride, RP-HPLC and Parenterals.- Visible Extractive Spectrophotometric Estimation of Teicoplanin in Bulk and in Pharmaceutical Formulations
Authors
1 Analytical Department, Vishnu Chemicals Limited, Hyderabad, IN
2 Department of Chemistry, S.K. University, Ananthpur, IN
3 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road #3, Banjara Hills, Hyderabad-500034, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 710-713Abstract
Three simple, accurate, rapid and sensitive methods (A, B and C) have been developed for the estimation of Teicoplanin in pharmaceutical dosage form. The Method A is based on reaction of Teicoplanin with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Teicoplanin with ferric chloride 2, 2’ bipyridyl to form blood colored chromogen. The Method C is based on the reduction of Ferric ions of the reagent Ferric chloride to Ferrous ions by the drug, which further in the presence of potassium ferricyanide as oxidizing agent produces blue colored chromogen measured at 700 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm, 520 nm and 720 nm respectively and obey the Beer’s law in the concentration range of 5-30 mcg/ml, 5-50 mcg/ml and. 1-10 mcg/ml respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Teicoplanin in bulk drug and in its formulations.Keywords
UV-Visible Spectrophotometry, Teicoplanin, Ferric Chloride, 1, 10-Phenanthroline, 2, 2’ Bipyridyl and Potassium Ferricyanide.- Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations
Authors
1 Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, IN
2 Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad, IN
3 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034., IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 4 (2019), Pagination: 1517-1520Abstract
Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Sofosbuvir in pharmaceutical dosage form. The Method A is based on reaction of Sofosbuvir with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Sofosbuvir with ferric chloride and 2,2'- bipyridyl to form a red colored ferroin complex measured at 520 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm and 520 nm respectively and obey the Beer’s law in the concentration range of 10-140 μg/mL and. 5-50 μg /mL respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Sofosbuvir in bulk drug and in its formulations.Keywords
UV-Visible Spectrophotometry, Sofosbuvir, Ferric Chloride, 1, 10-Phenanthroline, and 2, 2’ Bipyridyl.References
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